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Rev. argent. microbiol ; 44(2): 65-68, jun. 2012. ilus, graf, tab
Article in Spanish | LILACS | ID: lil-657613

ABSTRACT

Bacteria belonging to the family Chlamydiaceae cause a broad spectrum of diseases in a wide range of hosts, Including humans, other mammals and birds. However, very little is known about chlamydial infections in birds in our region. In the present study, we examined 28 clinically normal birds In illegal captivity that were confiscated in the province of Córdoba, Argentina. The objective was to detect Chlamydophila spp. in cloacal swabs by genetic analysis of the ompA gene. Nested-PCR of the ompA gene identified five samples as Chlamydophila pecorum and the sequence analysis demonstrated the presence of the ompA gene of C. pecorum In these birds. On the other hand, Chlamydophila psittaci was not detected. These birds could be either asymptomatic reservoirs or subclinical carriers of C. pecorum. This is the first report of the detection of C. pecorum in Argentina.


Las bacterias que pertenecen a la familia Chlamydiaceae causan un extenso espectro de enfermedades en una amplia gama de huéspedes, incluidos los seres humanos, otros mamíferos y aves. Sin embargo, se sabe muy poco acerca de las infecciones por clamidias en aves de nuestra reglón. Esta Investigación examinó 28 aves clínicamente normales mantenidas en cautiverio ¡legal, que fueron confiscadas en Córdoba, Argentina. El objetivo fue detectar Chlamydophila spp. en hisopados cloacales por análisis del gen ompA. La PCR anidada del gen ompA reveló la presencia de Chlamydophila pecorum en cinco muestras. El análisis de secuencias demostró la presencia del gen ompA de C. pecorum en estas aves. Por el contrario, Chlamydophila psittaci no se detectó. Estas aves pueden ser reservónos asintomáticos o portadores subclínlcos de C. pecorum. Este es el primer informe de la detección de C. pecorum en la Argentina.


Subject(s)
Animals , Bacterial Outer Membrane Proteins/genetics , Carrier State/veterinary , Chlamydophila Infections/veterinary , Chlamydophila/genetics , Genes, Bacterial , Passeriformes/genetics , Amino Acid Sequence , Argentina/epidemiology , Carrier State/epidemiology , Carrier State/microbiology , Chlamydophila Infections/epidemiology , Chlamydophila Infections/microbiology , Chlamydophila/classification , Cloaca/microbiology , Molecular Sequence Data , Phylogeny , Sequence Alignment , Sequence Homology, Amino Acid , Species Specificity
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